Installation

This script is designed to work on a Linux-based high performance computing (HPC) cluster, as used by most genomic centers. It will also work on a Mac or Linux based computer. The installation instructions are designed for installation on a HPC cluster with the assumption that the user does not have route access.

Dependencies

To use the identity.pl script you will need to install the following:

• Samtools
• BLAT
• Perl module: Bio::DB::Sam
• Perl module: Parallel::ForkManager

To install these dependencies see these links:

• Install Samtools
• Install BLAT
• Install Perl Modules

Installation

For the identity script to work it simply needs to be placed in a directory along with a list of SNPs for use in making SNP barcodes. This file is called 'SNP_list_all.txt' and can be downloaded here:

SNPlist

Testing identity.pl

To check that identity.pl is working use this command:

perl identity.pl

The output should look like this: